Pepsin show optimum activity acidic pH. The laboratory exercises presented here are designed to get students involved in nearly every as-pect of the teaching process. For pepsin, which is active in the stomach, the optimum pH is 2 (the pH of the stomach). of Vet. The aim of this activity is to investigate the effect of a reduction in enzyme concentration on the initial rate of reaction. 5. If the enzyme obeys Michaelis-Menten kinetics the kinetic parameters k 0 and k A often behave similarly. Add 2 ml of hydrogen peroxide to 5 test tubes 2. Optimum pH:is the pH at which the rate of reaction is maximum. Add 5 ml of water to an empty tube (this is the BLANK) To 3 separate tubes, add 2.5 ml of buffer pH 3, pH 5 or pH 7. The data indicated that the enzyme reaction did slow down in pH solutions other than 7. Effect of pH on CMCase activity: The pH of the growth medium affect the enzyme activity at various levels. Effect of temperature on enzymes The optimum (best) temperature for enzyme-controlled reactions is 370C (body temperature). An example of an acid on the pH scale is vinegar, which is typically assigned a value between two and three. effects of ph, temperature and enzyme to substrate ratio on the antioxidant activity of porcine hemoglobin hydrolysate prepared with pepsin QIAN SUN College of … The enormous catalytic activity of enzymes can perhaps best be expressed by a constant, k cat, that is variously referred to as the turnover rate, turnover frequency or turnover number.This constant represents the number of substrate molecules that can be converted to product by a single enzyme molecule per unit time (usually per minute or per second). Using clean test tubes, make six sets of pairs of original substrate and enzyme tubes for a total of 12 tubes or 6 pairs. Any change in this pH significantly affects the enzyme activity and/or the rate of reaction. Measure the time taken for amylase to completely break down starch, by withdrawing samples at 10 second intervals and noting the time at which the solution no longer gives a blue-black colour with iodine solution (but the iodine solution remains orange). 2. For every enzyme, there is an optimum pH value, at which the specific enzyme functions most actively. absorbance (enzyme activity) vs. enzyme concentration. 210 Effect of pH, heat treatments and proteinase K enzyme on the activity of Lactobacillus Acidophilus bacteriocin Marwa A. Saad1, Hamdi M. Abdelsamei1, Ekbal M.A.Ibrahim1, Adham M. Abdou1, Sobhy A. El Sohaimy2 1 Department of Food Control, Fac. This is graphically illustrated in Figure 14. The pancreas releases several enzymes, including proteases, which could be used to investigate the effect of enzyme … It was found that the value of the DH also increased when the temperature was increased from 30 to 40°C. Method. PH not only affects the activity of the enzyme, but also affects the charge and shape of the substrate, so that the substrate cannot bind to the active site, or cannot be catalyzed to form a product. In a narrow range of pH, the structural and morphological changes of enzymes and substrates may be reversible. To see the effect of pH on enzyme activity. Enzymes are affected by changes in pH. A pH level below or above seven indicates that the liquid is either an acid or a base. 2. Effects of pH. pH Effects on Enzyme Activity Structural Considerations: •Extreme pH changes will denature folded structure due to repulsive forces •Milder pH changes can dissociate oligomeric state of enzyme into inactive monomers •Therefore, important to be aware of protein stability issues due to pH conditions when characterizing enzyme activity Adding more acid does not affect the rate of activity of an enzyme. The Effect of pH, Concentration, and Temperature on Alkaline Phosphatase 2 the ALP enzyme is a neutral pH, the optimal temperature was 60°C, and an increase of concentration of the enzyme produced a greater rate of reaction. It is also expected for the reaction rates to drop as the pH is lowered or raised away from 7. the enzyme activity calculated from the change in absorbance at 530 nm. Measure and record the depth of the hydrogen peroxide. For many enzymes, this corresponds to pH values of around 7. It was expected that the reaction rate of the enzyme would be the highest in a solution of pH 7. Influence of the pH on enzyme assays. Effect of pH Each enzyme has an optimum pH. Enzyme activity and pH Now that you have established a set of conditions that causes hydrolysis of your added starch in 5 to 7 minutes at pH 7, you will test the effect of changing the pH. Because of this, most enzymes work within a narrow range of environmental/cellular conditions. As shown in Fig. There seems to be a common thermal effect on catalase, starch synthesis enzyme, and chlorophyll synthesis enzyme. Part I: The Effect of pH on Amylase Activity. You should find a pH optimum of 6 with the rate dropping off on either side. Enzyme Action—Effect of Enzyme Concentration, Temperature and pH on Catalase Activity Prelab Assignment Before coming to lab, read carefully the introduction and the procedures for parts I-III, and then answer the prelab questions at the end of this lab handout. Base your answer on the reactions shown in the graphic. about the relationship between pH and enzyme action? Shu et al., Effect of temperature, pH, enzyme to substrate ratio, substrate concentration 30 and time on the antioxidative activity of hydrolysates from goat milk casein by Alcalase and enzymatic reactions, and antioxidant enzymes and endogenous antioxidants in the body under synergy of has been removed (Murphy et al., 2011). 1. 40. At pH 8.6, for example, glucose oxidase activity was increased by a factor of three to 75O/, of its activity found at pH optimum due to trypsin activity. Effect of pH on amylase activity and stability To determine the optimum pH, amylase activity was measured at different pH values for 5 min at 30 …C. The Effect Of Low Ph On Enzyme Activity 1007 Words | 5 Pages. It was hypothesized that the reaction Each enzyme has a pH value that it works at with maximum efficiency called the optimal pH. A pH environment has a significant effect on an enzymes. It can affect the intramolecular forces and change the enzyme's shape -- potentially to the point where it is rendered ineffective. With these effects in mind, typical enzymes have a pH range in which they perform optimally. Introduction: Enzymes are catalysts or chemical agents that speed up chemical reactions without being consumed by the reaction (Reece et al. Effect of pH and Temperature on enzyme activity Effect of temperature can be studied by incubating the enzyme mixture containing enzyme, guaiacol and sodium-acetate buffer at different temperatures, i.e. The Effect of low pH on Enzyme Activity Name Institution Affiliation Outline Enzyme: Amylase Source: Barley Substrates: Starch from potatoes Iodine Method: Time-based experiment on the enzyme 's reaction to converting Starch into sugars by testing the amount of time in which a 2 cm3 of starch solution would be converted to sugars. enzymes into the small intestine. Ø A bell-shaped curve is obtained for the effect of pH and enzymatic activity. All the factors that affect enzyme kinetics are constant except pH where it varies in each tube Time = 5 min Temp= 37 oC [S] = 0.05M Add to each tube (Test and Blank): - 0.5 ml of Corresponding pH buffer - 0.5 ml of pNPP - 0.5 ml MgCl 2 - 5 ml water Method: Place in … All enzymes work best at a neutral pH. Part C- Effect of temperature on enzyme activity ; Part D- Effect of pH on enzyme activity; Photo Gallery; Enzyme Lab: Procedure. Description. If the pH is lower or higher than the optimal pH, the enzyme activity decreases until it stops working. The students are asked to prepare the project by reading the primary literature on the physiological role of enzymes as well as their indus- The test tube was then immersed in a water bath at 25°C for 5 min. Effect of pH on Enzyme Activity. the enzyme activity calculated from the change in absorbance at 530 nm. The objective of this study was to investigate how changes in soil pH affect the N2O and N2 emissions, denitrification activity, and size of a denitrifier community. On the other hand, trypsin works in the small intestine, parts of which have a pH of around 7.5. Cells were grown in a chemostat under hemin-excess conditions over a range of pH values; stable growth was observed only between pH 6.7 and 8.3, with the maximum … The reaction studied was the breakdown of hydrogen peroxide catalyzed by the enzyme peroxidase. At metal concentrations of 0.6mM, Zn and Ni reduced the enzyme activity by 25-30% under optimal pH conditions (pH … Kinetics. This research focused on the effect of changes in pH on enzyme activity. The NAD malic enzyme shows a pH optimum of 6.7 when complexed to Mg 2+ and NAD + but shifts to 7.0 when the catalytically competent enzyme-substrate (E-S) complex forms upon binding malate −2.This is characteristic of an induced conformational change. An enzyme exhibits maximum activity over the narrow pH range in which a molecule exists in its properly charged form. To this solution add 0.5M (2.8053 grams) KOH drop by drop till the pH is set to 6.8. An example of a base is ammonia, which has a pH value between 11 and 12. Immunoblotting for effect of (a) 24 h phytochemical treatment (control, 5, 10 and 20 mM as indicated) and protein expression of phase II enzymes in the 1-month-old cell model. It was hypothesized that the reaction Any change in this pH significantly affects the enzyme activity and/or the rate of reaction. Enzyme Activity of Salivary Amylase Page 4 Set up and label a new 24‐well plate for three trials: one each at pH 5, … 1: pH for Optimum Activity. 50. To know more about the relation between pH and enzymes, and/or the effect of pH on enzymes… Ø Each enzyme will have an optimum pH where the velocity of the reaction is maximum. Add 2.5 ml of starch (substrate) to each of these tubes (excluding BLANK) Add 2 drops of iodine to each tube and mix: Blank, pH 3, pH 5, pH 7. B. Above or below an enzyme’s optimum pH, its activity is lower. Tube 3; add 3 drops of water Introduction. Immunoblotting for effect of (a) 24 h phytochemical treatment (control, 5, 10 and 20 mM as indicated) and protein expression of phase II enzymes in the 1-month-old cell model. In this experiment you will To establish enzyme importance in disease conditions. Enzyme Cut-Out Activity Directions AP help for Enzyme cut and paste activity Quick Guide to Calculating Enzyme Activity Effect of Temperature on Enzyme Activity | GCSE Biology (9-1) | kayscience.com Webinar | Healthy Living In Difficult Times Restriction enzymes Effect of pH on Enzyme Activity | GCSE Biology (9-1) | kayscience.com The more acidic pH added to the solution did not increase the rate of the reaction. The pancreas releases several enzymes, including proteases, which could be used to investigate the effect of enzyme … Both simultane- Optimal pH. Alternative hypothesis - If there is a higher pH level, the enzyme catalytic rate will be higher and if the pH level is lower than the enzyme catalytic rate will be lower. x. The NAD malic enzyme shows a pH optimum of 6.7 when complexed to Mg 2+ and NAD + but shifts to 7.0 when the catalytically competent enzyme-substrate (E-S) complex forms upon binding malate −2.This is characteristic of an induced conformational change. properties of the enzyme. 10. However, at pH 9.5 there was a marked reduction in CAT activity, which was similar to the result seen with SOD at this extreme pH. Both enzymes are stable, at least up to pH 9.7, under the conditions used. Methods/Materials In a test tube 3 mL of water, 1 mL of lactose and 1 mL of a specific buffer solution (7.0 pH as the control; An enzyme concentration at 1.5% was subsequently used in the study of the effect of the other parameters. C. Enzymes function only in a pH range of 4.0 to 5.5. Enzyme Catalyst lab Abstract: This laboratory explores the affects pH has on a reaction rate. Add cell wall to the buffers and run the assays. Label 3 small paper cups as follows: pH 1,pH 7, pH 9, and dilute catalase into the appropriate paper cup as directed below: a. pH 1: 5 mL catalase + 5 mL pH 3 buffer b. Experiment 2: The Effect of pH on Amylase Enzyme Activity Part 2: Amylase Reactions 1. 20. x An effect on enzyme substrate complex. Likewise, as the pH +is raised, the enzyme will lose H ions and eventually lose its active shape. It has an optimum pH of about 1.5. A. As the temperature increases, the rate of reaction increases. Most intracellular enzymes exhibit optimal activity at pH values between 5-9. For pH stability, the enzyme solution at a desired pH, was kept at 4 …C for 24 h, and then activity was measured at the … Ionic detergents, such as (SDS) sodium dodecylsulfate, are potent protein denaturants that will bind to proteins and unfold their native forms. The reaction studied was the breakdown of hydrogen peroxide catalyzed by the enzyme peroxidase. 2.2.1. BACKGROUND. that the enzyme activity will be slowed or stopped in pH solutions that were either acidic or basic. As the pH value is increased above or decreased below the optimum pH the enzyme activity decreases. At very acidic and alkaline pH values the shape of the enzyme is altered so that it is no longer complementary to its specific substrate. This effect can be permanent and irreversible and is called denaturation. Effect of pH on enzyme activity. The reaction rate of the enzyme will then be measured and graphed. In this experiment, the reaction tubes will have identical enzyme and substrate concentrations, temperature, etc., but the pH will vary from one reaction 2 Food Technology Department arid land research institute city of scientific Research and technology In exercise 4.1 we used a pH 7 buffer and ran the experiment at room temperature or approximately 20°C. Three trials were run at pH levels of 6, 7 and 8. It was also expected that the reaction rate would decrease as the pH deviated from 7. 2, polyphenol oxidase activity was pH-dependent, with complete inactivation at 2.0 ≤ pH > 10. A reversed effect was measured during urease activity. Highest activity measured was 8.67 U/ml. In our predictions, we believed the catalyse would have a higher reaction rate in a more acidic environment. See the appendix for easy instructions of how to mix up the phosphate buffers. It is important to study the effect of pH on enzyme activity so it can be learned when amylase will function with maximum efficiency. Enzyme activity is at its maximum value at the optimum pH. Therefore, it is possible that one or several matrix components could have a restrictive effect on detergent AAMY activity in the qualitative assay at 40°C but not at 60°C. 2010). Tube 1; add 10 drops of HCL 3. 55°C is the critical temperature Graphical and computer analysis of K, and Vm,, data, obtained with these assays as a function of pH, suggest the participation in isomerase catalysis of two ionizable groups in the enzyme with average pK values of 6.75 and 9.3, at 30”. increase in enzyme activity from the pH 7.0 to the pH 9.0 condition. The conclusion of the experiment was that pH, temperature, and concentration have an effect on the enzyme activity of ALP. Highest enzyme activity (2.295 units/ liter) on wheat straw at pH … The studies on pH stability showed that the enzyme was stable in a range of pH 7.0–10.0 and the effect of the inhibitors showed it to be possibly an alkaline protease. These results show that PPO activity is more effectively inhibited in an acid than in an alkaline pH with PPO activity ranging from 0.13 to 13.81 U.mL −1. The efficiency of an enzyme is largely influenced by the pH value of its surroundings. This is because the charge of its component amino acids changes with the change in the pH value. Each enzyme becomes active at a certain pH level. Enzymes are potent catalysts. enzyme structure or function will destroy their biological activity. The optimum pH of a particular enzyme corresponds to the pH of its natural environment. Enzymes are very significant for biochemical reactions, they enable reactions to speed up and. In this system the salt also abolished binding of the enzyme to the lipid droplets, whereas in the Intralipid system most of the enzyme remained bound even at 1 M NaCl. Med., Benha Univ., Egypt. 30. Table 3.7. Mix up phosphate buffers at different pH2 (3,4,5,6,7,8,9). The extracted enzyme will be mixed with a solution of known pH. enzyme activity in the crude extract was 40 C at a pH between 8.0 and 9.0. Stability studies revealed temperatures around 40–60 C. Tube 2; add 3 drops of HCL 4. 2. What is the effect of temperature on enzyme activity? Each of you will identify the enzyme(s) you received by determining the effect of pH on enzymatic activity. But very high temperatures denature enzymes. (2014). Extremely high or low pH values generally result in complete loss of activity for most enzymes. 60-----Enzymes work best at or around body temperature (37 degrees C) Enzymes work slower at colder temperatures Enzymes denature at higher temperatures rendering them inactive. The aim of this activity is to investigate the effect of a reduction in enzyme concentration on the initial rate of reaction. enzymes into the small intestine. EFFECT OF PH The rate of almost all enzyme-catalyzed reactions exhibits a significant dependence on hydrogen ion concentration. On the other hand, at pH 6.0, i.e. The activity of enzymes depends strictly on the pH in the assay mixture. The more basic pH that was added to the enzyme reaction caused the overall reaction to speed up. Temp. The students are asked to prepare the project by reading the primary literature on the physiological role of enzymes as well as their indus- pH is a measure of how acidic or alkaline a solution is. Effect of pH on enzyme activity. enzyme, and some will receive both enzymes in one solution. Enzyme activity was higher on sixth day (Figure 5). The results obtained in the quantitative pH assays suggest that the activity of the enzyme from detergent C is higher at a neutral pH but is also consistent at alkaline pHs. For every enzyme, there is an optimum pH value, at which the specific enzyme functions most actively. Many enzymes perform optimally in the neutral pH range and 3. The rates of enzyme-catalysed reactions vary with pH and often pass through a maximum as the pH is varied. Most enzymes work at neutral pH 7.4. Trypsin's optimum pH is about 8. __pH_____ 3. 3. Factors affecting enzyme function pH changes in pH adds or remove H+ disrupts bonds, disrupts 3D shape disrupts attractions between charged amino acids affect 2° & 3° structure denatures protein optimal pH? objective: See the effect that pH has on the rate of Enzyme activity. D. The activity of an enzyme is affected by pH. What is the effect of pH on enzyme activity? However, pH 5 had seen to be favourable for the production of maximum enzyme activity at all the substrates and pH studied. Enzyme reactions depend strongly on pH and temperature. Presented below are notes and comments regarding the laboratory material and preparation for the laboratory exercise. provide an alternative reaction pathway. Pepsin works in the highly acidic conditions of the stomach. As the pH is lowered an enzyme will tend to gain H+ ions, and eventually enough side chains will be affected so that the enzyme’s shape is disrupted. Enzyme Catalyst lab Abstract: This laboratory explores the affects pH has on a reaction rate. pH hypothesis: Null hypothesis - The pH level will have no effect on the enzyme catalytic rate. Since enzymes are proteins, their secondary and tertiary structures are affected by temperature, pH, and the presence of heavy metal ions. At higher or lower pH, the rate of an enzymatic reaction decrease. when enzyme concentration was increased from 1.0% to 1.5% but became constant at concentration exceeding 1.5%. Most enzymes are proteins that function … All three of the investigated heavy metals inhibited the enzyme activity in acetate buf- fer. Ø Hydrogen ions in the medium influence the ionic charges on the amino acids in the enzymes particularly on the active site. Enzyme. Effect of phytochemical treatment on phase II enzyme protein expression. Biochemistry Activity #9 page 6 4. The activities of most enzymes follow a bell-shaped curve, increasing from zero in the strong acid region up to a maximum value, and decreasing to zero to … EFFECT OF pH ON THE ENZYME-CATALYZED REACTION In the previous experiment, the conditions in the control and mixed tubes were identical except for the concentration of the enzyme. Part D- Effect of pH on Enzyme Activity - Understanding Enzymes. Reagents: x 0.5% Starch in 20 to 100 mill molar phosphate buffer (pH 6.8) x Preparation of phosphate buffer: Dissolve 0.2M (2.7218 grams) of KH 2PO 4 in 100ml of distilled water. A change in pH can alter the bonds of the 3-dimensional shape of an enzyme and cause the enzyme to change shape, which may slow or … of the enzyme (part (b) of Figure 18.14 "Temperature and pH … __temperature_____ 2. In particular, changes in pH, temperature and salinity will significantly affect enzyme function but altering protein structure. The most favorable pH value - the point where the enzyme is most active - is known as the optimum pH. Description. For example, pepsin works at a low pH, i.e, it is highly acidic, while trypsin works at a high pH, i.e, it is basic. The slope of the V max or V max /K m profiles is steeper on the alkaline side of the pH optimum. The direct dependence of the enzyme becomes obvious, when the enzyme activity is measured at the respective pH or temperature, while preincubation for a distinct time yields the stability of the enzyme against these influences. The median value of this pH range is called the optimum pH The pH at which a particular enzyme exhibits maximum activity. Investigating Enzymes - Effect of Temperature on Enzyme Activity Teacher’s Guide This guide is a continuation of the previous laboratory documents. Enzyme activity PPO activity was determined according to the method modified from TAN et al. The Effect Of Low Ph On Enzyme Activity 1007 Words | 5 Pages. Obtain 1 paper cup of 40mL 3% H 2O 2. most human enzymes = pH 6-8 depends on localized conditions pepsin (stomach) = pH … of pH and buffer type. 1 In Exercise 4.1 you learned a method of measuring the reaction as peroxidase converts hydrogen peroxide to oxygen and water. Enzyme denaturation can be caused by high temperatures, extremes in pH, organic solvents, and repetitive cycles of freezing and thawing. A volume of 5.5 mL of 0.2M phosphate buffer (pH 6.0) and 1.5 mL of 0.2 M pyrocatechol solution were added into a test tube. The pH at which the rate or a suitable parameter is a maximum is called the pH optimum and the plot of rate or parameter against pH is called a pH profile. Both factors were found to exert a strong effect on the activity of the enzyme. Thus pre- incubation of the enzymes at pH 9.2 and 9.7 (I = 0.06, T = 37 "C) for 1 min before assaying the remaining activity at pH7.3 gave the same results as pre- 1. Since the pH of the gingival crevice increases from below neutrality in health to above pH 8 in disease, we decided to investigate the effect of environmental pH on the growth and enzyme activity of Bacteroides gingivalis W50. pH is a measure of how acidic or alkaline a solution is. Determining the Effect if pH on Enzymatic Activity Procedure: 1. and pH and the effect of surfactants and oxidants on AAMY activity. Gain or loss of critical charged groups adversely affects substrate binding and thus will retard or abolish catalysis. __enzyme/substrate concentration_____ a. (4). All Absorbance readings today will be taken at 405 nm, the absorption peak of p- pH H 2O 2 Depth Time Rate Rate: Class Ave. 3 5 7 9 11 . The low activity of T4 lysozyme at alkaline pH is not caused by denaturation of the en- zyme at high pH. The maximum PPO enzyme activity from buriti pulp observed at pH 7.0 was 14.69 U.mL −1, using catechol as substrate. Each enzyme has an optimal pH at which it is the most active or effective. The laboratory exercises presented here are designed to get students involved in nearly every as-pect of the teaching process. Enzyme activity is closely associated with the structure of an enzyme, so any change in the secondary or tertiary structure leads to a change in enzyme activity. Amylase Activity (%) 75 % . Abstract. Effect of phytochemical treatment on phase II enzyme protein expression. The effect of pH on enzyme activity pH It is expected that the reaction rate will be highest for the enzymes mixed with pH 7. 0. A similar picture was obtained with respect to effects of pH. pH is also a factor in the stability of enzymes. Finally, we tested the effect of pH on the enzyme with pH 7, pH 1 and pH 11. temperatures on the bromelain within pineapple juice. below the optimum, it increased by a factor of two to about 800/, due to urease activity. Quite obviously, a pH of 9.5 was near the tolerance limit for H. verticillata. To see the effect an of an enzyme inhibitor on enzyme activity. Enzyme activity is at its maximum value at the optimum pH. You can measure the effect of pH on the rate of the lysosome reaction. Investigating the effect of pH on amylase activity. The Effect of Temperature on Enzyme Function The enzyme activity was highest at neutral pH (7) and showed only 1/3 enzyme activity at pH 11while pH of 1 showed no enzyme activity. Somewhat similarly, the maximum accumulation of MDA was observed Set up a Bunsen burner, heatproof mat, tripod and gauze. The activity of enzymes is affected by temperature and pH. The Effect of pH on Lactase S0410 Objectives/Goals The objective of this project is to determine the pH level of an environment in which lactase, an enzyme that breaks down lactose to galactose and glucose, is most active. The Effect of low pH on Enzyme Activity Name Institution Affiliation Outline Enzyme: Amylase Source: Barley Substrates: Starch from potatoes Iodine Method: Time-based experiment on the enzyme 's reaction to converting Starch into sugars by testing the amount of time in which a 2 cm3 of starch solution would be converted to sugars. NOTE: If necessary, review the directions for the use of the Spectronic 20 in Laboratory 1. was demonstrated for the range of pH 6.0 to 9.5. Procedure: What is the effect of pH on enzyme activity ? Enzymatic activity of protease in fruit juice on gelatin and the reaction of different PH and. The slope of the V max or V max /K m profiles is steeper on the alkaline side of the pH optimum. 6. Take two cuvettes from the Containers shelf and place them Part 1: Preparing the Reaction Solutions onto the workbench. It is important to study the effect of pH on enzyme activity so it can be learned when amylase will function with maximum efficiency. The effect of temperature on enzyme activity b. Abstract. To know more about the relation between pH and enzymes, and/or the effect of pH on enzymes… Fill the first cuvette with 4.5 mL of 6 M HCl and the second 1. Factors affecting Enzyme Activity Enzymes are affected by 1. In contrast, 0.5 M or more NaCl virtually abolished the enzyme activity in the milk system. However, the hydrolysis at and pH and the effect of surfactants and oxidants on AAMY activity. Three trials were run at pH levels of 6, 7 and 8. In Part D, we approached understanding the effect of different pH levels on the liver's catalase. Enzyme Cut-Out Activity Directions AP help for Enzyme cut and paste activity Quick Guide to Calculating Enzyme Activity Effect of Temperature on Enzyme Activity | GCSE Biology (9-1) | kayscience.com Webinar | Healthy Living In Difficult Times Restriction enzymes Effect of pH on Enzyme Activity | GCSE Biology (9-1) | kayscience.com pH at which an enzyme’s activity is greatest. 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